sybr green quantitative polymerase chain reaction pcr mix Search Results


sybr quantitative reverse transcription polymerase chain reaction (qrt-pcr) kit  (Yeasen Biotechnology)
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Yeasen Biotechnology sybr quantitative reverse transcription polymerase chain reaction (qrt-pcr) kit
Verification of significant DEGs between Hp-positive or Hp-negative gastric cancer cell lines. (A,B,C,D,E) AGS cells were treated with Hp strain 26695 for 4 h, and the TNFRSF7, LTF, GM-CSF receptor (CSF2RB), PTPRC and EMP3 expressions were analyzed by quantitative reverse <t>transcription</t> <t>polymerase</t> chain reaction assay (qRT-PCR). (F,G,H,I,J) BGC-823 cells were infected with Hp strain 26695 for 4 h, and MAPK7, IGFBP2, ANG, PDGFA, and DTR mRNA levels were analyzed by qRT-PCR assay. No additional treatment was considered the control group. aP<0.001 compared with the no treatment group. DEGs, differentially expressed genes; Hp, Helicobacter pylori; TNFRSF7, tumor necrosis factor receptor superfamily member 7; LTF, lactotransferrin; PTPRC, protein tyrosine phosphatase receptor type C; EMP3, epithelial membrane protein 3; MAPK7, Mitogen-activated protein kinase 7; IGFBP2, insulin-like growth factor binding protein 2; ANG, angiotensin; PDGFA, platelet-derived growth factor subunit A; DTR, diphtheria toxin receptor.
Sybr Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt Pcr) Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green quantitative polymerase chain reaction (pcr) amplifications kit  (tiangen biotech co)
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tiangen biotech co sybr green quantitative polymerase chain reaction (pcr) amplifications kit
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Green Quantitative Polymerase Chain Reaction (Pcr) Amplifications Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green mrna quantitative real-time polymerase chain reaction (pcr  (Ribobio co)
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Ribobio co sybr green mrna quantitative real-time polymerase chain reaction (pcr
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Green Mrna Quantitative Real Time Polymerase Chain Reaction (Pcr, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green i fluorescent quantitative polymerase chain reaction (pcr)  (Eppendorf AG)
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Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Green I Fluorescent Quantitative Polymerase Chain Reaction (Pcr), supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr primers for sybr green quantitative polymerase chain reaction  (Cowin Biosciences)
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Cowin Biosciences pcr primers for sybr green quantitative polymerase chain reaction
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Pcr Primers For Sybr Green Quantitative Polymerase Chain Reaction, supplied by Cowin Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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one-step sybr green quantitative real-time polymerase chain reaction (qrt-pcr) kit  (GenStar Biosolutions)
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GenStar Biosolutions one-step sybr green quantitative real-time polymerase chain reaction (qrt-pcr) kit
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
One Step Sybr Green Quantitative Real Time Polymerase Chain Reaction (Qrt Pcr) Kit, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green based one-tube quantitative reverse transcription polymerase chain reaction (qrt-pcr)  (Qiagen)
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Qiagen sybr green based one-tube quantitative reverse transcription polymerase chain reaction (qrt-pcr)
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Green Based One Tube Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt Pcr), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green quantitative real-time polymerase chain reaction (pcr)  (DBI Bioscience)
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DBI Bioscience sybr green quantitative real-time polymerase chain reaction (pcr)
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Green Quantitative Real Time Polymerase Chain Reaction (Pcr), supplied by DBI Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr fluorescent quantitative polymerase chain reaction (fq-pcr) kit  (Promega)
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Promega sybr fluorescent quantitative polymerase chain reaction (fq-pcr) kit
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Fluorescent Quantitative Polymerase Chain Reaction (Fq Pcr) Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abi quantitative pcr instrument and a sybr green reverse transcription-polymerase chain reaction kit  (Beijing CWBio)
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Beijing CWBio abi quantitative pcr instrument and a sybr green reverse transcription-polymerase chain reaction kit
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Abi Quantitative Pcr Instrument And A Sybr Green Reverse Transcription Polymerase Chain Reaction Kit, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitect one-step sybr green quantitative reverse transcription-polymerase chain reaction (qrt-pcr) master mix kit  (Qiagen)
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Qiagen quantitect one-step sybr green quantitative reverse transcription-polymerase chain reaction (qrt-pcr) master mix kit
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Quantitect One Step Sybr Green Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt Pcr) Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green-based quantitative fluorescent polymerase chain reaction (pcr) method  (Qiagen)
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Qiagen sybr green-based quantitative fluorescent polymerase chain reaction (pcr) method
Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time <t>polymerase</t> chain reaction <t>(PCR).</t> Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.
Sybr Green Based Quantitative Fluorescent Polymerase Chain Reaction (Pcr) Method, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sybr green-based quantitative fluorescent polymerase chain reaction (pcr) method/product/Qiagen
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Image Search Results


Verification of significant DEGs between Hp-positive or Hp-negative gastric cancer cell lines. (A,B,C,D,E) AGS cells were treated with Hp strain 26695 for 4 h, and the TNFRSF7, LTF, GM-CSF receptor (CSF2RB), PTPRC and EMP3 expressions were analyzed by quantitative reverse transcription polymerase chain reaction assay (qRT-PCR). (F,G,H,I,J) BGC-823 cells were infected with Hp strain 26695 for 4 h, and MAPK7, IGFBP2, ANG, PDGFA, and DTR mRNA levels were analyzed by qRT-PCR assay. No additional treatment was considered the control group. aP<0.001 compared with the no treatment group. DEGs, differentially expressed genes; Hp, Helicobacter pylori; TNFRSF7, tumor necrosis factor receptor superfamily member 7; LTF, lactotransferrin; PTPRC, protein tyrosine phosphatase receptor type C; EMP3, epithelial membrane protein 3; MAPK7, Mitogen-activated protein kinase 7; IGFBP2, insulin-like growth factor binding protein 2; ANG, angiotensin; PDGFA, platelet-derived growth factor subunit A; DTR, diphtheria toxin receptor.

Journal: Journal of Gastrointestinal Oncology

Article Title: Helicobacter pylori -induced protein tyrosine phosphatase receptor type C as a prognostic biomarker for gastric cancer

doi: 10.21037/jgo-21-305

Figure Lengend Snippet: Verification of significant DEGs between Hp-positive or Hp-negative gastric cancer cell lines. (A,B,C,D,E) AGS cells were treated with Hp strain 26695 for 4 h, and the TNFRSF7, LTF, GM-CSF receptor (CSF2RB), PTPRC and EMP3 expressions were analyzed by quantitative reverse transcription polymerase chain reaction assay (qRT-PCR). (F,G,H,I,J) BGC-823 cells were infected with Hp strain 26695 for 4 h, and MAPK7, IGFBP2, ANG, PDGFA, and DTR mRNA levels were analyzed by qRT-PCR assay. No additional treatment was considered the control group. aP<0.001 compared with the no treatment group. DEGs, differentially expressed genes; Hp, Helicobacter pylori; TNFRSF7, tumor necrosis factor receptor superfamily member 7; LTF, lactotransferrin; PTPRC, protein tyrosine phosphatase receptor type C; EMP3, epithelial membrane protein 3; MAPK7, Mitogen-activated protein kinase 7; IGFBP2, insulin-like growth factor binding protein 2; ANG, angiotensin; PDGFA, platelet-derived growth factor subunit A; DTR, diphtheria toxin receptor.

Article Snippet: The total RNA isolation kit, first-strand cDNA synthesis kit, and SYBR quantitative reverse transcription polymerase chain reaction (qRT-PCR) kit were obtained from Yeasen Biotechnology (Shanghai, China).

Techniques: Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Infection, Control, Membrane, Binding Assay, Derivative Assay

Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time polymerase chain reaction (PCR). Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.

Journal: Oncology Research

Article Title: IGF-I Induces Epithelial-to-Mesenchymal Transition via the IGF-IR–Src–MicroRNA-30a–E-Cadherin Pathway in Nasopharyngeal Carcinoma Cells

doi: 10.3727/096504016X14648701447931

Figure Lengend Snippet: Inhibition of miR-30a repressed IGF-I-induced EMT in NPC cells. (A) The serum-starved CNE2 cells were treated with IGF-I (100 ng/ml) for 48 h. The expression of miR-30a was analyzed by real-time polymerase chain reaction (PCR). Data are mean ± SD from three independent experiments. Control group as reference. *IGF-I untreated versus IGF-I treated, p < 0.05. (B) miR-30a inhibitor was transfected into CNE2 cells for 48 h. The expression of miR-30a was analyzed by real-time PCR. Data are mean ± SD from three independent experiments. Control group as reference. *Control transfected versus miR-30a inhibitor transfected, p < 0.05. (C, D) The serum-starved cells were transfected with or without the miR-30a inhibitor for 48 h followed by IGF-I (100 ng/ml) stimulation for 48 h. Cell lysates were collected for Western blot analysis. Photos were taken at 20× magnification. E-cad, E-cadherin.

Article Snippet: Expression of miRNA-30a (miR-30a) was detected by SYBR Green quantitative polymerase chain reaction (PCR) amplifications kit (Tiangen Biotech) and measured by the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, USA).

Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Control, Transfection, Western Blot